IjongweSebenzisa
Le mveliso ilungele ukuchongwa ngokomgangatho we-COVID-19.Ibonelela ngoncedo ekuxilongeni usulelo lwenoveli coronavirus.
USHWANKATHELO
Inoveli coronaviruses yeyodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla.Ngokuqhelekileyo abantu basengozini.Okwangoku, izigulana ezosulelwe yinoveli coronavirus ngoyena mthombo wosulelo;abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela yi-1 ukuya kwiintsuku ze-14, ubukhulu becala iintsuku ezi-3 ukuya kwezi-7.Iimpawu eziphambili ziquka umkhuhlane, ukukhathala kunye nokukhwehlela okomileyo.Ukuxinana kweempumlo, iimpumlo, umqala obuhlungu, i-myalgia kunye ne-diarrhea zifumaneka kwiimeko ezimbalwa.
UMGAQO
Ikhithi yovavanyo iqulethe imicu yovavanyo emibini:
Kwenye yazo, i-burgundy colored conjugate pad equlathe iNovel coronavirus recombinant envelope antigens edityaniswe negolide yeColloid (iNovel coronavirus conjugates), 2) umtya we-nitrocellulose membrane oqulethe imigca emibini yovavanyo (imigca ye-IgG kunye ne-IgM) kunye nomgca wolawulo (umgca we-C1) .
Umgca we-IgM uqatywe ngaphambili kunye ne-antibody ye-IgM ye-mouse, umgca we-IgG uqatywe nge-Mouse anti-Human IgG antibody.Xa umthamo owaneleyo wesampulu yovavanyo ukhutshwa kwisampulu yequla lesixhobo sovavanyo, umboniso ufuduka ngentshukumo yecapillary kwisixhobo.I-IgM ye-anti-Novel coronavirus, ukuba ikhona kumzekelo, iya kuzibophelela kwi-Novel coronavirus conjugates.
I-immunocomplex ke ibanjwe yi-reagent efakwe ngaphambili kwi-IgM band, yenza umgca we-IgM ombala we-burgundy, obonisa i-Novel coronavirus IgM isiphumo sovavanyo oluhle.I-IgG ye-anti-Novel coronavirus ukuba ikhona kumzekelo iya kubophelela kwi-Novel coronavirus conjugates.I-immunocomplex ke ibanjwe yi-reagent ecatyiswe kumgca we-IgG, yenze umgca we-IgG we-burgundy onemibala, ebonisa i-Novel coronavirus IgG isiphumo sovavanyo oluhle.Ukungabikho kwayo nayiphi na imigca ye-T (i-IgG kunye ne-IgM) ibonisa umphumo ongalunganga.Ukuze usebenze njengolawulo lwenkqubo, umgca wombala uya kuhlala ubonakala kummandla wolawulo obonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
Kwelinye icwecwe, umtya wovavanyo uqulunqwe ngala malungu alandelayo: angala iphedi yesampulu, iphedi yereagent, inwebu yokusabela, kunye nephedi yokufunxa.I-reagent pad iqulethe i-colloidal-gold conjugated kunye ne-monoclonal antibodies ngokuchasene ne-nucleocapsid protein ye-SARS-CoV-2;inwebu yokusabela iqulethe izilwa-buhlungu zesibini ze-nucleocapsid protein ye-SARS-CoV-2.Uluhlu olupheleleyo luzinze ngaphakathi kwisixhobo seplastiki.Xa isampuli ifakwe kwisampuli kakuhle, i-conjugates eyomisiwe kwi-reagent pad iyachithwa kwaye ifuduke kunye nesampuli.Ukuba i-antigen ye-SARS-CoV-2 iveza kwisampulu, ikhompleksi eyenziwe phakathi kwe-anti-SARS-2 conjugate kunye nentsholongwane iya kuthinjwa zii-anti-SARS-2 ze-monoclonal antibodies ezigqunywe kumgca wovavanyo (T).Ukungabikho komgca we-T kubonisa umphumo ongalunganga.Ukuze usebenze njengolawulo lwenkqubo umgca obomvu uya kuhlala ubonakala kwingingqi yolawulo (C2) ebonisa ukuba umthamo ofanelekileyo wesampuli wongeziweyo kwaye i-membrane wicking yenzeke.
Ixesha lokuposa: Apr-01-2021