China Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit abavelisi kunye nababoneleli |Yinye

Inoveli yeCoronavirus (SARS-CoV-2) Multiplex yeXesha lokwenyani PCR Kit

Inoveli yeCoronavirus (i-SARS-CoV-2) iMultiplex yeXesha lokwenyani leKit yePCR eFakelwe umfanekiso

Inkcazelo emfutshane:

I-REF	500190	Inkcazo	Iimvavanyo ezingama-96/Ibhokisi
Umgaqo wokufumanisa	I-PCR	Imizekelo	Iimpumlo / i-Nasopharyngeal swab

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Le khithi yePCR enovakalelo kakhulu, elungele ukusetyenziswa iyafumaneka kwifomati ye-lyophilized (inkqubo yokumisa umkhenkce) yokugcina ixesha elide.Ikiti inokuthuthwa kwaye igcinwe kwiqondo lokushisa kwaye izinzile unyaka omnye.Ityhubhu nganye ye-premix iqulethe zonke ii-reagents ezifunekayo kwi-PCR amplification, kuquka i-Reverse-transcriptase, i-Taq polymerase, i-primers, i-probes, kunye ne-dNTPs substrates.Ifuna kuphela ukongeza i-13ul yamanzi adibeneyo kunye ne-5ul ekhutshwe i-template ye-RNA, ngoko inokuqhutywa kwaye ikhuliswe kwizixhobo ze-PCR.

Umatshini we-qPCR kufuneka adibaneezi mfuno zilandelayo:

1. Fit 8 strip PCR tube volume 0.2 ml
2. Yiba nemijelo engaphezulu kwesine yokubona:

Umjelo	Uvuyo (nm)	Ukukhutshwa (nm)	Iidayi eziCalibrated kwangaphambili
1.	470	525	FAM, SYBR Green I
2	523	564	VIC, HEX, TET, JOE
3.	571	621	I-ROX, I-TEXAS-RED
4	630	670	CY5

PCR-amaqonga:
7500Inkqubo yePCR yexesha lokwenyani, iBiorad CF96, iCycler iQ™ Inkqubo yokubona iPCR yexesha elililo, Stratagene Mx3000P, Mx3005P

Ubunzima bokuthuthwa kwekhonkco elibandayo leInoveli yeCoronavirus ye-nucleic acid yokufumanisa ireagent

Xa ii-reagents zokubona i-nucleic acid eqhelekileyo zithuthwa kumgama omde, i-(-20±5) ℃ ukugcinwa kwekhonkco elibandayo kunye nokuthuthwa kuyafuneka ukuze kuqinisekiswe i-bioactive ye-enzyme kwii-reagents zihlala zisebenza.Ukuqinisekisa ukuba izinga lokushisa lifikelela kumgangatho, iikhilogram eziliqela zomkhenkce owomileyo ziyafuneka kwibhokisi nganye ye-nucleic acid test reagent nangaphantsi kwe-50g, kodwa inokuhlala iintsuku ezimbini okanye ezintathu kuphela.Ngokombono wokuziqhelanisa noshishino, obona bunzima beerejenti ezikhutshwe ngabavelisi bungaphantsi kwe-10% (okanye ngaphantsi kakhulu kweli xabiso) lwesikhongozeli.Uninzi lobunzima buvela kumkhenkce owomileyo, iipakethe zomkhenkce kunye neebhokisi zegwebu, ngoko ke iindleko zothutho ziphezulu kakhulu.

Ngo-Matshi ka-2020, i-COVID-19 yaqala ukuqhambuka kwinqanaba elikhulu phesheya, kwaye imfuno yeNoveli yeCoronavirus i-nucleic acid yokubona i-reagent inyuke kakhulu.Ngaphandle kweendleko eziphezulu zokuthumela ii-reagents kwikhonkco elibandayo, abaninzi abavelisi basenakho ukuyamkela ngenxa yobuninzi kunye nenzuzo ephezulu.

Nangona kunjalo, ngokuphuculwa kwemigaqo-nkqubo yesizwe yokuthumela ngaphandle kwiimveliso ezichasene nobhubhane, kunye nokuphuculwa kolawulo lwesizwe lokuhamba kwabantu kunye nokuhanjiswa, kukho ukongezwa kunye nokungaqiniseki kwixesha lothutho lwe-reagents, okubangele iingxaki ezibalaseleyo zemveliso ezibangelwayo. ngezothutho.Ixesha elongeziweyo lezothutho (ixesha lokuhamba malunga nesiqingatha senyanga lixhaphake kakhulu) likhokelela ekungaphumeleli kwemveliso rhoqo xa imveliso ifikelela kumxhasi.Oku kuye kwawakhathaza kakhulu amashishini athumela ngaphandle kwe-nucleic acid.

Itekhnoloji yeLyophilized ye-PCR reagent yanceda ukuthuthwa kweInoveli yeCoronavirus yokufumanisa i-reagent ye-nucleic acid kwihlabathi liphela

I-PCR reagents ye-lyophilized inokuthuthwa kwaye igcinwe kwiqondo lokushisa, elingenako ukunciphisa iindleko zokuthutha kuphela, kodwa liphinde ligweme iingxaki zekhwalithi ezibangelwa yinkqubo yokuthutha.Ngoko ke, i-lyophilizing i-reagent yindlela efanelekileyo yokusombulula ingxaki yokuthumela ngaphandle.

I-Lyophilization ibandakanya ukukhenkceza isisombululo kwisimo esiqinileyo, kwaye emva koko i-sublimate kwaye ihlukanise umphunga wamanzi phantsi kwemeko ye-vacuum.I-solute eyomileyo ihlala kwisitya kunye nokubunjwa okufanayo kunye nomsebenzi.Xa kuthelekiswa neereagents zolwelo eziqhelekileyo, eyona nto igcweleyo i-lyophilized Novel Coronavirus nucleic acid yokufumanisa ireagent eveliswe yiLiming Bio inezi mpawu zilandelayo:

Uzinzo lobushushu olomeleleyo kakhulu:inokuthi ngonyango lokuma kwi-56 ℃ kwiintsuku ze-60, kwaye i-morphology kunye nokusebenza kwe-reagent ihlala ingatshintshi.
Ugcino lobushushu obuqhelekileyo kunye nothutho:akukho mfuneko yekhonkco elibandayo, akukho mfuneko yokugcina kwiqondo lokushisa eliphantsi ngaphambi kokungatywinwa, ukukhulula ngokupheleleyo indawo yokugcina ebandayo.
Ilungele ukusetyenziswa:lyophilizing yawo onke amacandelo, akukho mfuneko yoqwalaselo lwenkqubo, ukuphepha ukulahleka kwamacandelo kunye ne-viscosity ephezulu efana ne-enzyme.
Iithagethi ezininzi kumbhobho omnye:ufuzo ekujoliswe kulo lugubungela inoveli coronavirus ORF1ab gene, N, S ukunqanda genovariation yentsholongwane.Ukuze kuncitshiswe ubuxoki obungalunganga, ijini ye-RNase P yomntu isetyenziswa njengolawulo lwangaphakathi, ukuze kuhlangatyezwane nemfuno yekliniki yolawulo lomgangatho wesampulu.